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PPAR delta antibody

Anti-PPAR delta antibody used in Western Blot (WB). GTX79186
Anti-PPAR delta antibody used in Western Blot (WB). GTX79186
Anti-PPAR delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79186

Cat. No. GTX79186

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, FACS, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PPAR delta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250-1:750
ICC/IF 1-2 μg/ml
IHC-Fr 1:200
FACS 3-5 μg/10⁶ cells
IHC Assay dependent
Not tested in other applications.

Product Note

This antibody does not detect PPAR alpha or PPAR gamma.

PROPERTIES

Form

Liquid

Buffer

buffer, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues M(1) E Q P Q E E T P E A R E E(14) C of mouse PPAR delta.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11172030

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxisome proliferator-activated receptor delta , Pparb

Background

transcription factor of the nuclear hormone receptor superfamily; may mediate the intestinal expression of target genes implicated in lipid metabolism [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-PPAR delta antibody used in Western Blot (WB). GTX79186

GTX79186 WB Image

WB analysis of 25 ug of NIH-3T3 (Lane 1), PC12 (Lane 2), and K562 (Lane 3) cell lysates using GTX79186 PPAR delta antibody.
Dilution : 1:2000 (NIH-3T3 and K562) and 1:1000 (PC12)

Anti-PPAR delta antibody used in Western Blot (WB). GTX79186

GTX79186 WB Image

WB analysis of whole cell extracts (20 μg lysate) of NIH/3T3 (Lane 1), THP-1 (Lane 2), PC-3 (Lane 3), PC-12 (Lane 4), SH-SY5Y (Lane 5), U 2-OS (Lane 6), HCT 116 (Lane 7), K562 (Lane 8), A-431 (Lane 9) and Jurkat (Lane 10) using GTX79186 PPAR delta antibody.
Dilution : 1:250-1:750

Anti-PPAR delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79186

GTX79186 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX79186 PPAR delta antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399