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PPHLN antibody

Anti-PPHLN antibody used in IHC (Paraffin sections) (IHC-P). GTX87045
Anti-PPHLN antibody used in Western Blot (WB). GTX87045

Cat. No. GTX87045

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all PPHLN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human PPHLN (71-120).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CR , HSPC206 , HSPC232 , PPHLN1 , periphilin 1

Cellular Localization

Nucleus,Cytoplasm,Chromosome

Background

The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPHLN antibody used in IHC (Paraffin sections) (IHC-P). GTX87045

GTX87045 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX87045 PPHLN antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PPHLN antibody used in Western Blot (WB). GTX87045

GTX87045 WB Image

WB analysis of COLO and 293 cell lysates using GTX87045 PPHLN antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399