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PPIH antibody

Anti-PPIH antibody used in Western Blot (WB). GTX118224
Anti-PPIH antibody used in IHC (Paraffin sections) (IHC-P). GTX118224
Anti-PPIH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118224

Cat. No. GTX118224

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PPIH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Zebrafish, Bovine, Cat, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPIH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616830

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidylprolyl isomerase H , CYP-20 , CYPH , SnuCyp-20 , USA-CYP

Cellular Localization

Nucleus speckle , Cytoplasm

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPIH antibody used in Western Blot (WB). GTX118224

GTX118224 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX118224 diluted at 1:1000

Anti-PPIH antibody used in IHC (Paraffin sections) (IHC-P). GTX118224

GTX118224 IHC-P Image

PPIH antibody detects PPIH protein at nucleus on Mahlarvu xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Mahlarvu xenograft.
PPIH antibody (GTX118224) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PPIH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118224

GTX118224 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPIH(GTX118224) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169