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PROM2 antibody [14D1]

Overexpression
Anti-PROM2 antibody [14D1] used in Western Blot (WB). GTX83792
Anti-PROM2 antibody [14D1] used in IHC (Paraffin sections) (IHC-P). GTX83792
Anti-PROM2 antibody [14D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83792
Anti-PROM2 antibody [14D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83792
Anti-PROM2 antibody [14D1] used in Western Blot (WB). GTX83792

Cat. No. GTX83792

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 14D1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($519)
See all PROM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2500-1:5000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human PROM2 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prominin 2 , PROML2

Cellular Localization

Apical cell membrane

Background

This gene encodes a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PROM2 antibody [14D1] used in Western Blot (WB). GTX83792

GTX83792 WB Image

WB analysis of various cell lines using GTX83792 PROM2 antibody [14D1].
Loading : 35 ug per lane
Dilution : 1:200

Anti-PROM2 antibody [14D1] used in IHC (Paraffin sections) (IHC-P). GTX83792

GTX83792 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX83792 PROM2 antibody [14D1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PROM2 antibody [14D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83792

GTX83792 ICC/IF Image

ICC/IF analysis of COS7 cells using GTX83792 PROM2 antibody [14D1].

Anti-PROM2 antibody [14D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83792

GTX83792 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PROM2 plasmid using GTX83792 PROM2 antibody [14D1].

Anti-PROM2 antibody [14D1] used in Western Blot (WB). GTX83792

GTX83792 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PROM2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83792 PROM2 antibody [14D1].
Loading : 5 ug per lane

REFERENCE

Application Reference

Ziller N et al. Cells 2020; 9 (10) Sex Differences in Diabetes- and TGF-β1-Induced Renal Damage.
Application : ICC/IF, IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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Application Reference
Ziller N et al. Cells 2020; 9 (10) Sex Differences in Diabetes- and TGF-β1-Induced Renal Damage.
Application : ICC/IF, IHC-P
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519