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PROX1 antibody

Orthogonal
Anti-PROX1 antibody used in Western Blot (WB). GTX129143
Anti-PROX1 antibody used in Western Blot (WB). GTX129143
Anti-PROX1 antibody used in Western Blot (WB). GTX129143
Anti-PROX1 antibody used in Western Blot (WB). GTX129143
Anti-PROX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129143

Cat. No. GTX129143

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PROX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

fetal mouse forebrain , mouse eye , mouse hippocampus , HepG2 nuclear extract

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PROX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885908

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prospero homeobox 1

Cellular Localization

Nucleus

Background

May play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons.

Database

Research Area

DATA IMAGES

Anti-PROX1 antibody used in Western Blot (WB). GTX129143

GTX129143 WB Image

Orthogonal

HepG2 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PROX1 antibody (GTX129143) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PROX1 antibody used in Western Blot (WB). GTX129143

GTX129143 WB Image

PROX1 antibody detects PROX1 protein by western blot analysis.
A. 50 μg fetal mouse forebrain lysate/extract
7.5 % SDS-PAGE
PROX1 antibody (GTX129143) dilution: 1:1000

Anti-PROX1 antibody used in Western Blot (WB). GTX129143

GTX129143 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PROX1 antibody (GTX129143) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PROX1 antibody used in Western Blot (WB). GTX129143

GTX129143 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PROX1 antibody (GTX129143) diluted at 1:500.

Anti-PROX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129143

GTX129143 ICC/IF Image

PROX1 antibody detects PROX1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PROX1 protein stained by PROX1 antibody (GTX129143) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169