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PRX I antibody

Anti-PRX I antibody used in Western Blot (WB). GTX15571
Anti-PRX I antibody used in Western Blot (WB). GTX15571
Anti-PRX I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15571
Anti-PRX I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15571

Cat. No. GTX15571

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all PRX I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000 - 1:5,000
ICC/IF 1:250
IHC Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Product Note

This antibody is specific for Prx 1 and shows no cross-reactivity with other Prx isoforms.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues L(103) V S D P K R T I A Q D(114) of human Peroxiredoxin 1.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_371537

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 1 , MSP23 , NKEF-A , NKEFA , PAG , PAGA , PAGB , PRX1 , PRXI , TDPX2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-PRX I antibody used in Western Blot (WB). GTX15571

GTX15571 WB Image

WB analysis of 25 ug of various whole cell lysates using GTX15571 PRX I antibody.
Dilution : 1:1000

Anti-PRX I antibody used in Western Blot (WB). GTX15571

GTX15571 WB Image

WB analysis of whole cell extracts (30 μg lysate) of Hep G2 (Lane 1), Raji (Lane 2) and Ramos (Lane 3) using GTX15571 PRX I antibody.
Dilution : 0.5 μg/ml

Anti-PRX I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15571

GTX15571 ICC/IF Image

ICC/IF analysis of T-47D cells using GTX15571 PRX I antibody. Panel e shows the no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

Anti-PRX I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15571

GTX15571 ICC/IF Image

ICC/IF analysis of TSU-Pr1 cells using GTX15571 PRX I antibody.

REFERENCE

Application Reference

Wulff JE et al. J Am Chem Soc 2007; 129 (46) : 14444-51 The natural product avrainvillamide binds to the oncoprotein nucleophosmin.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399