APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT1F8 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PRDX5 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peroxiredoxin 3 , AOP1 , HBC189 , MER5 , PRDX3 , PRO1748 , SP22 , prxIII , PRX III , Peroxiredoxin 3
Cellular Localization
Mitochondrion,Cytoplasm
Background
This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. [provided by RefSeq, Oct 2014]
Database
Research Area
DATA IMAGES
![Anti-PRX III antibody [AT1F8] used in Western Blot (WB). GTX57660](/templates/default/js/lightbox/images/loading.gif "Anti-PRX III antibody [AT1F8] used in Western Blot (WB). GTX57660")
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GTX57660 WB Image
WB analysis of various samples using GTX57660 PRX III antibody.
Lane 1 : Recombinant Human PRX III protein (50 ng)
Lane 2 : HeLa whole cell lysate (40 μg)
Lane 3 : HepG2 whole cell lysate (40 μg)
Lane 4 : TF1 whole cell lysate (40 μg)
Lane 5 : U87-MG whole cell lysate (40 μg)
Lane 6 : Raji whole cell lysate (40 μg)
Lane 7 : 293T whole cell lysate (40 μg)
Lane 8 : Jurkat whole cell lysate (40 μg)
Lane 9 : MCF-7 whole cell lysate (40 μg)
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GTX57660 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57660 PRX III antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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REFERENCE
There are currently no references for PRX III antibody [AT1F8] (GTX57660). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PRX III antibody [AT1F8] (GTX57660). Be the first to share your experience with this product.
![Anti-PRX III antibody [AT1F8] used in Western Blot (WB). GTX57660](/upload/website/prouct_img/small/GTX57660/GTX57660_20200204_WB_w_23061123_213.webp "Anti-PRX III antibody [AT1F8] used in Western Blot (WB). GTX57660")
![Anti-PRX III antibody [AT1F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57660](/upload/website/prouct_img/small/GTX57660/GTX57660_20200204_IF_w_23061123_399.webp "Anti-PRX III antibody [AT1F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57660")
![Anti-PRX III antibody [AT1F8] used in Western Blot (WB). GTX57660](/upload/website/prouct_img/normal/GTX57660/GTX57660_20200204_WB_w_23061123_213.webp "Anti-PRX III antibody [AT1F8] used in Western Blot (WB). GTX57660")
![Anti-PRX III antibody [AT1F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57660](/upload/website/prouct_img/normal/GTX57660/GTX57660_20200204_IF_w_23061123_399.webp "Anti-PRX III antibody [AT1F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57660")