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PSMA3 antibody

Anti-PSMA3 antibody used in Western Blot (WB). GTX113529
Anti-PSMA3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113529
Anti-PSMA3 antibody used in Western Blot (WB). GTX113529

Cat. No. GTX113529

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PSMA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , Molt-4

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSMA3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037763

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome subunit alpha 3 , HC8 , PSC3

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSMA3 antibody used in Western Blot (WB). GTX113529

GTX113529 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
12% SDS PAGE
GTX113529 diluted at 1:1000

Anti-PSMA3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113529

GTX113529 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft , using 20S Proteasome alpha3 (GTX113529) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PSMA3 antibody used in Western Blot (WB). GTX113529

GTX113529 WB Image

Proteasome 20S alpha 3 antibody detects PSMA3 protein by Western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 3 antibody (GTX113529) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169