APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , 293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.96 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PSMA5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951507
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proteasome subunit alpha 5 , PSC5 , ZETA
Cellular Localization
Cytoplasm , Nucleus
Background
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX103845 IHC-P Image
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Proteasome 20S alpha 5(GTX103845) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103845 WB Image
PSMA5 antibody detects PSMA5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMA5 antibody (GTX103845) diluted by 1:1000.
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GTX103845 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Proteasome 20S alpha5(GTX103845) antibody at 1:200 dilution.
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GTX103845 WB Image
Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX103845 diluted at 1:5000
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GTX103845 IHC-P Image
PSMA5 antibody detects PSMA5 protein at cytoplasm and nucleus in human hepatocellular carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
PSMA5 antibody (GTX103845) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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REVIEW
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