GeneTex

  • Country / Location Selection

United States (US)

PSRC1 antibody

Overexpression
Anti-PSRC1 antibody used in Western Blot (WB). GTX128047
Anti-PSRC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX128047
Anti-PSRC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128047
Anti-PSRC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128047
Anti-PSRC1 antibody used in Western Blot (WB). GTX128047

Cat. No. GTX128047

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PSRC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

human PSRC1-transfected 293T cells , 293T , A431

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSRC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885690

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proline and serine rich coiled-coil 1 , DDA3 , FP3214

Cellular Localization

Cytoplasm

Background

This gene encodes a proline-rich protein. Studies of the related mouse gene suggest that this gene is regulated by p53 and may participate in p53-mediated growth suppression. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSRC1 antibody used in Western Blot (WB). GTX128047

GTX128047 WB Image

PSRC1 antibody detects PSRC1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSRC1 antibody (GTX128047) diluted by 1:1000.

Anti-PSRC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX128047

GTX128047 IHC-Fr Image

PSRC1 antibody detects PSRC1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: PSRC1 stained by PSRC1 antibody (GTX128047) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PSRC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128047

GTX128047 IHC-P Image

PSRC1 antibody detects PSRC1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PSRC1 stained by PSRC1 antibody (GTX128047) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PSRC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128047

GTX128047 ICC/IF Image

PSRC1 antibody detects PSRC1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSRC1 protein stained by PSRC1 antibody (GTX128047) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PSRC1 antibody used in Western Blot (WB). GTX128047

GTX128047 WB Image

Overexpression

PSRC1 antibody detects PSRC1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human PSRC1-transfected 293T cells
12 % SDS-PAGE
PSRC1 antibody (GTX128047) dilution: 1:10000

REFERENCE

Application Reference

Han R et al. J Cancer 2023; 14 (17) : 3321-3334 High Expression of PSRC1 Predicts Poor Prognosis in Lung Adenocarcinoma.
R Han et al. J Cancer 2023; High Expression of PSRC1 Predicts Poor Prognosis in Lung Adenocarcinoma
Application : IHC-P
Luo T et al. Gut Microbes 2022; 14 (1) : 2077602 Deficiency of PSRC1 accelerates atherosclerosis by increasing TMAO production via manipulating gut microbiota and flavin monooxygenase 3.
Application : WB
Reactivity : Mouse
Nehlig A et al. Cell Mol Life Sci 2020; (Epub) Reciprocal regulation of Aurora kinase A and ATIP3 in the control of metaphase spindle length.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for PSRC1 antibody (GTX128047). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169