PTCH1 antibody [5c7]

Cat. No. GTX83771

Reference 5

Host	Mouse
Clonality	Monoclonal
Clone Name	5c7
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($539)

See all PTCH1 products

View product citations for antibody GTX83771 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
ICC/IF	1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW

161 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 122-436 of human PTCH1 (NP_000255) produced in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10729289

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

patched 1 , BCNS , NBCCS , PTC , PTC1 , PTCH

Cellular Localization

Cell membrane

Background

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.

Database

Gene ID: 5727 PTCH1
UniProt: Q13635 PTCH1

Research Area

DATA IMAGES

GTX83771 WB Image

WB analysis of HEK293T cells transfected with PTCH1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83771 PTCH1 antibody [5c7].
Loading : 5 ug per lane

GTX83771 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX83771 PTCH1 antibody [5c7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83771 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX83771 PTCH1 antibody [5c7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83771 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX83771 PTCH1 antibody [5c7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83771 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83771 PTCH1 antibody [5c7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83771 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX83771 PTCH1 antibody [5c7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83771 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83771 PTCH1 antibody [5c7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83771 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83771 PTCH1 antibody [5c7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83771 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX83771 PTCH1 antibody [5c7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83771 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83771 PTCH1 antibody [5c7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83771 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX83771 PTCH1 antibody [5c7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83771 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PTCH1 plasmid using GTX83771 PTCH1 antibody [5c7].

REFERENCE

Application Reference

Zambrano-Rom?n M et al. J Clin Lab Anal 2024; 38 (3) : e25010 PTCH1 gene variants rs357564, rs2236405, rs2297086 and rs41313327, mRNA and tissue expression in basal cell carcinoma patients from Western Mexico.

Liu B et al. Cell Cycle 2020; 19 (24) : 3546-3562 Promoting roles of long non-coding RNA FAM83H-AS1 in bladder cancer growth, metastasis, and angiogenesis through the c-Myc-mediated ULK3 upregulation.

Application : WB

Reactivity : Human

Yao F et al. Oncol Lett 2019; 18 (5) : 4825-4833 Primary impact of Gli1 on radioresistance in esophageal cancer.

Application : WB

Reactivity : Human

Qi X et al. Science 2018; (Epub) Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex.

Application : WB

Reactivity : Human

Fleet A et al. J Biol Chem 2016; (Epub) Activities of the Cytoplasmic Domains of Patched-1 Modulate but are not Essential for Regulation of Canonical Hedgehog Signaling.

Application : WB

Reactivity : Human

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REVIEW

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Application Reference

Zambrano-Rom?n M et al. J Clin Lab Anal 2024; 38 (3):e25010 PTCH1 gene variants rs357564, rs2236405, rs2297086 and rs41313327, mRNA and tissue expression in basal cell carcinoma patients from Western Mexico.

Liu B et al. Cell Cycle 2020; 19 (24):3546-3562 Promoting roles of long non-coding RNA FAM83H-AS1 in bladder cancer growth, metastasis, and angiogenesis through the c-Myc-mediated ULK3 upregulation.

Application : WB

Reactivity : Human

Yao F et al. Oncol Lett 2019; 18 (5):4825-4833 Primary impact of Gli1 on radioresistance in esophageal cancer.

Application : WB

Reactivity : Human

Qi X et al. Science 2018; (Epub) Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex.

Application : WB

Reactivity : Human

Fleet A et al. J Biol Chem 2016; (Epub) Activities of the Cytoplasmic Domains of Patched-1 Modulate but are not Essential for Regulation of Canonical Hedgehog Signaling.

Application : WB

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package	List Price ($)
100 μl	$ 539
Qty

PTCH1 antibody [5c7]

Cat. No. GTX83771

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83771 WB Image

GTX83771 IHC-P Image

GTX83771 IHC-P Image

GTX83771 IHC-P Image

GTX83771 IHC-P Image

GTX83771 IHC-P Image

GTX83771 IHC-P Image

GTX83771 IHC-P Image

GTX83771 IHC-P Image

GTX83771 IHC-P Image

GTX83771 IHC-P Image

GTX83771 ICC/IF Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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