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PTCH1 antibody

Anti-PTCH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64432
Anti-PTCH1 antibody used in Western Blot (WB). GTX64432
Anti-PTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64432
Anti-PTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64432
Anti-PTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64432
Anti-PTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64432
Anti-PTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64432

Cat. No. GTX64432

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PTCH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1214-1312 of human PTCH1 (NP_000255.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

patched 1 , BCNS , NBCCS , PTC , PTC1 , PTCH

Cellular Localization

Cell membrane

Background

This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-PTCH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64432

GTX64432 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64432 PTCH1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PTCH1 antibody used in Western Blot (WB). GTX64432

GTX64432 WB Image

WB analysis of various sample lysates using GTX64432 PTCH1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64432

GTX64432 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX64432 PTCH1 antibody.
Dilution : 1:100

Anti-PTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64432

GTX64432 IHC-P Image

IHC-P analysis of human breast tissue using GTX64432 PTCH1 antibody.
Dilution : 1:100

Anti-PTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64432

GTX64432 IHC-P Image

IHC-P analysis of mouse embryos tissue using GTX64432 PTCH1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64432

GTX64432 IHC-P Image

IHC-P analysis of mouse embryos tissue using GTX64432 PTCH1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64432

GTX64432 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX64432 PTCH1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399