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PTF1A antibody, Internal

Anti-PTF1A antibody, Internal used in Western Blot (WB). GTX89052
Anti-PTF1A antibody, Internal used in Western Blot (WB). GTX89052

Cat. No. GTX89052

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all PTF1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1.5μg/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89052-PEP).

Predict Reactivity

Rat, Zebrafish, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-WTDEKQLKEQN, from the internal region of the protein sequence according to NP_835455.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pancreas associated transcription factor 1a , PACA , PAGEN2 , PTF1-p48 , bHLHa29 , p48

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PTF1A antibody, Internal used in Western Blot (WB). GTX89052

GTX89052 WB Image

WB analysis of human pancreas lysate (Lane A) using GTX89052 PTF1A antibody, Internal preincubated with immunogen peptide (Lane B).
Dilution: 0.5μg/ml
Loading: 35μg per lane

Anti-PTF1A antibody, Internal used in Western Blot (WB). GTX89052

GTX89052 WB Image

WB analysis of various sample lysates using GTX89052 PTF1A antibody, Internal preincubated with immunogen peptide.
Dilution: 0.5-1μg/ml
Loading: 35μg per lane

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399