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PTF1A antibody

Orthogonal
Anti-PTF1A antibody used in Western Blot (WB). GTX129525
Anti-PTF1A antibody used in Western Blot (WB). GTX129525
Anti-PTF1A antibody used in Western Blot (WB). GTX129525

Cat. No. GTX129525

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PTF1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

AsPC-1

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PTF1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886025

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pancreas associated transcription factor 1a , PACA , PAGEN2 , PTF1-p48 , bHLHa29 , p48

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PTF1A antibody used in Western Blot (WB). GTX129525

GTX129525 WB Image

PTF1A antibody detects PTF1A protein by western blot analysis.
A. 50 μg AsPC-1 whole cell lysate/extract
10 % SDS-PAGE
PTF1A antibody (GTX129525) dilution: 1:500

Anti-PTF1A antibody used in Western Blot (WB). GTX129525

GTX129525 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PTF1A antibody (GTX129525) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTF1A antibody used in Western Blot (WB). GTX129525

GTX129525 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTF1A antibody (GTX129525) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169