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PTH1R antibody

Anti-PTH1R antibody used in Western Blot (WB). GTX79394
Anti-PTH1R antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79394
Anti-PTH1R antibody used in IHC (Paraffin sections) (IHC-P). GTX79394

Cat. No. GTX79394

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all PTH1R products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000
ICC/IF 1:250
IHC-P 1:2000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the C-terminal amino acids E(573)EASGPERPPALLQEEWETVM(593) of PTHR1

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11174591

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

parathyroid hormone 1 receptor , EKNS , PFE , PTHR , PTHR1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-PTH1R antibody used in Western Blot (WB). GTX79394

GTX79394 WB Image

WB analysis of whole cell extracts (30ug) of Hep G2 (Lane 1) and U-2 OS (Lane 2) using GTX79394 PTH1R antibody.
Dilution : 1:5000

Anti-PTH1R antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79394

GTX79394 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX79394 PTH1R antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

Anti-PTH1R antibody used in IHC (Paraffin sections) (IHC-P). GTX79394

GTX79394 IHC-P Image

IHC-P analysis of human kidney tissue using GTX79394 PTH1R antibody.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399