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PTTG1IP antibody

Anti-PTTG1IP antibody used in Western Blot (WB). GTX55274
Anti-PTTG1IP antibody used in IHC (Paraffin sections) (IHC-P). GTX55274
Anti-PTTG1IP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55274

Cat. No. GTX55274

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of PTTG1IP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PTTG1 interacting protein , C21orf1 , C21orf3 , PBF

Cellular Localization

Membrane,Cytoplasm,Nucleus

Background

This gene encodes a single-pass type I integral membrane protein, which binds to pituitary tumor-transforming 1 protein (PTTG1), and facilitates translocation of PTTG1 into the nucleus. Coexpression of this protein and PTTG1 induces transcriptional activation of basic fibroblast growth factor. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013]

Database

  • Gene ID: 754 PTTG1IP
  • UniProt: P53801 PTTG1IP

DATA IMAGES

Anti-PTTG1IP antibody used in Western Blot (WB). GTX55274

GTX55274 WB Image

WB analysis of human colon (A), Raw264.7 (B) whole cell lysates using GTX55274 PTTG1IP antibody.

Anti-PTTG1IP antibody used in IHC (Paraffin sections) (IHC-P). GTX55274

GTX55274 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55274 PTTG1IP antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-PTTG1IP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55274

GTX55274 ICC/IF Image

ICC/IF analysis of formalin-fixed A549 cells using GTX55274 PTTG1IP antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399