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PXR antibody, Internal

Anti-PXR antibody, Internal used in Western Blot (WB). GTX81102
Anti-PXR antibody, Internal used in Western Blot (WB). GTX81102
Anti-PXR antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81102
Anti-PXR antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81102
Anti-PXR antibody, Internal used in Flow cytometry (FACS). GTX81102

Cat. No. GTX81102

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all PXR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 100-127 amino acids from the Central region of human NR1I2.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11170361

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 1 group I member 2 , BXR , ONR1 , PAR , PAR1 , PAR2 , PARq , PRR , PXR , SAR , SXR

Cellular Localization

Nucleus

Background

This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PXR antibody, Internal used in Western Blot (WB). GTX81102

GTX81102 WB Image

WB analysis of HeLa whole cell lysate using GTX81102 PXR antibody, Internal.
Loading : 20 μg per lane
Dilution : 1:1000

Anti-PXR antibody, Internal used in Western Blot (WB). GTX81102

GTX81102 WB Image

WB analysis of HL-60 cell lysate (35ug/lane) using GTX81102 PXR antibody, Internal.

Anti-PXR antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81102

GTX81102 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX81102 PXR antibody, Internal.

Anti-PXR antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81102

GTX81102 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX81102 PXR antibody, Internal.
Green : PXR
Red : Actin filament
Blue : cell nucleus

Anti-PXR antibody, Internal used in Flow cytometry (FACS). GTX81102

GTX81102 FACS Image

FACS analysis of HL-60 cells using GTX81102 PXR antibody, Internal.
Top histogram : negative control
Bottom histogram : HL-60 cells

REFERENCE

Application Reference

Hao Li et al. Bioorg Chem 2021; 109 : 104661 Deciphering structural bases of intestinal and hepatic selectivity in targeting pregnane X receptor with indole-based microbial mimics.
Application : IHC-P
Reactivity : Human
Rachel E Etherington et al. FASEB 2019; (Epub) Bile acid receptor agonists in primary biliary cholangitis: regulation of the cholangiocyte secretome and downstream T cell differentiation.
Application : IHC-P
Reactivity : Human
He Y et al. Comp Biochem Physiol C Toxicol Pharmacol 2018; 214 : 43359 Relationship between CYP3A29 and pregnane X receptor in landrace pigs: Pig CYP3A29 has a similar mechanism of regulation to human CYP3A4.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399