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Paxillin (non-phospho Tyr118) antibody [GT722]

KO/KD Orthogonal
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633707
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

Cat. No. GTX633707

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT722

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Paxillin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HeLa , A431 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (non-phospho Tyr118). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888372

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

GTX633707 WB Image

KO/KD Orthogonal

Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed effect is based on the publication: PMID: 16705174

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633707

GTX633707 ICC/IF Image

Paxillin (non-phospho Tyr118) antibody [GT722] detects Paxillin (non-phospho Tyr118) protein at focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin (non-phospho Tyr118) stained by Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

GTX633707 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

GTX633707 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000.

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

GTX633707 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

GTX633707 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations