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Phospholipase D2 (phospho Tyr169) antibody

Anti-Phospholipase D2 (phospho Tyr169) antibody used in IHC (Paraffin sections) (IHC-P). GTX55368
Anti-Phospholipase D2 (phospho Tyr169) antibody used in Western Blot (WB). GTX55368

Cat. No. GTX55368

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Phospholipase D2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 169 (E-N-Y(p)-L-N) derived from human Phospholipase D2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase D2 , PLD1C

Cellular Localization

Membrane

Background

The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-Phospholipase D2 (phospho Tyr169) antibody used in IHC (Paraffin sections) (IHC-P). GTX55368

GTX55368 IHC-P Image

IHC-P analysis of human brain tissue using GTX55368 Phospholipase D2 (phospho Tyr169) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Phospholipase D2 (phospho Tyr169) antibody used in Western Blot (WB). GTX55368

GTX55368 WB Image

WB analysis of extracts from Jurkat cells treated with TNF using GTX55368 Phospholipase D2 (phospho Tyr169) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399