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Phosphothreonine antibody [18F6]

Anti-Phosphothreonine antibody [18F6] used in Western Blot (WB). GTX22286
Anti-Phosphothreonine antibody [18F6] used in ELISA (ELISA). GTX22286
Anti-Phosphothreonine antibody [18F6] used in ELISA (ELISA). GTX22286
Anti-Phosphothreonine antibody [18F6] used in Western Blot (WB). GTX22286

Cat. No. GTX22286

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 18F6

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Species independent
Package
50 μl ($399)
See all Phosphothreonine products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:4000-1:20000
Not tested in other applications.

Product Note

Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.

PROPERTIES

Form

Liquid

Buffer

Steriled-filtered ascites

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

Phosphothreonine conjugated KLH.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.

Research Area

DATA IMAGES

Anti-Phosphothreonine antibody [18F6] used in Western Blot (WB). GTX22286

GTX22286 WB Image

Mab anti-phosphothreonine antibody (clone 18F6, GTX22286) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1,000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using 1:2,500 dilution Goat-a-Mouse IgG [H&L] secondary antibody and visualization using the OdysseyR Infrared Imaging System developed by LI-COR.

Anti-Phosphothreonine antibody [18F6] used in ELISA (ELISA). GTX22286

GTX22286 ELISA Image

ELISA results of Mab anti-phosphothreonine antibody (GTX22286) tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1μg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) antibody and TMB substrate were used for detection.

Anti-Phosphothreonine antibody [18F6] used in ELISA (ELISA). GTX22286

GTX22286 ELISA Image

ELISA analysis of BSA-conjugated pT, pY and pS using serially diluted GTX22286 Phosphothreonine antibody [18F6].
Coating : 0.1 μg
Dilution : 1:1000

Anti-Phosphothreonine antibody [18F6] used in Western Blot (WB). GTX22286

GTX22286 WB Image

WB analysis of EGF-treated A431 whole cell lysate using GTX22286 Phosphothreonine antibody [18F6].
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399