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Phosphotyrosine antibody [13F9]

Orthogonal
Anti-Phosphotyrosine antibody [13F9] used in Western Blot (WB). GTX22287
Anti-Phosphotyrosine antibody [13F9] used in ELISA (ELISA). GTX22287
Anti-Phosphotyrosine antibody [13F9] used in ELISA (ELISA). GTX22287
Anti-Phosphotyrosine antibody [13F9] used in Western Blot (WB). GTX22287

Cat. No. GTX22287

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 13F9

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Species independent
Package
50 μl ($399)
See all Phosphotyrosine products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:4000-1:20000
Not tested in other applications.

Product Note

Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.

PROPERTIES

Form

Liquid

Buffer

Steriled-filtered ascites

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

Phosphotyrosine conjugated KLH.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation

Research Area

DATA IMAGES

Anti-Phosphotyrosine antibody [13F9] used in Western Blot (WB). GTX22287

GTX22287 WB Image

Western Blot of Mouse anti-phosphotyrosine antibody (clone 13F9) (GTX22287). Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after coommassie staining. Load: 10 μg per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody:Goat-a-Mouse IgG [H&L] secondary antibody diluted 1:2,500 for 30' at room temperature. Block: 5% BLOTTO overnight at 4ºC.

Anti-Phosphotyrosine antibody [13F9] used in ELISA (ELISA). GTX22287

GTX22287 ELISA Image

ELISA of Mouse anti-phosphotyrosine antibody (GTX22287). Antigen: BSA conjugates of pT, pY and pS. Coating amount: 0.1 μg per well. Primary antibody: Phosphotyrosine antibody at 1:1000. Dilution series (X-axis): 2-fold. Secondary antibody: HRP conjugated Gt-a-Mouse IgG H&L secondary antibody at 1:20,000. Substrate: TMB

Anti-Phosphotyrosine antibody [13F9] used in ELISA (ELISA). GTX22287

GTX22287 ELISA Image

ELISA analysis of BSA-conjugated pT, pY and pS using serially diluted GTX22287 Phosphotyrosine antibody [13F9].
Coating : 0.1 μg
Dilution : 1:1000

Anti-Phosphotyrosine antibody [13F9] used in Western Blot (WB). GTX22287

GTX22287 WB Image

Orthogonal

WB analysis of various samples using GTX22287 Phosphotyrosine antibody [13F9].
Lane 1 : Phosphorylated EGFR in A431 whole cell lysate
Lane 2 : Unstimulated A431 whole cell lysate
Loading : 10 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399