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Ppa2 (S. pombe) antibody

Anti-Ppa2 (S. pombe) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64145
Anti-Ppa2 (S. pombe) antibody used in Western Blot (WB). GTX64145

Cat. No. GTX64145

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Schizosaccharomyces pombe
Package
100 μl ($399)
See all Ppa2 products

APPLICATION

Application Note

Immunoblotting (dilution: 1:1000)

Calculated MW

36 kDa. ( Note )

Product Note

The antibody recognized both Ppa1 and Ppa2 polypeptides in S. pombe because of their high amino acid similarity (~80% identity)

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

Recombinant C-terminal polypeptide (26kDa) of S. pombe Ppa2

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-Ppa2 (S. pombe) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64145

GTX64145 ICC/IF Image

Cellular location of Ppa1 and Ppa2. Indirect immunofluorescence microscopy of ppa2 deletion, wild type (WT), and wild-type overexpressing ppa2 (nmt-ppa2, 18hr) was done, using anti-ppa2 antibody (right); The same cells stained by DAPI are also shown (left). Immunofluorescence was hardly detected in ppa2 cells, whereas cytoplasmic immunofluorescence was abundant in wild-type cells. Wild-type cells carrying nmt-ppa2 plasmid overexpress Ppa2 protein in the absence of thiamine for 18 hr. Immunofluorescence was enhanced further in the cytoplasm, often accumulated at the nuclear periphery or within restricted domains. The deformation of chromosomal DNA was also visible in overexpressed cells. Bar, 10um

Anti-Ppa2 (S. pombe) antibody used in Western Blot (WB). GTX64145

GTX64145 WB Image

Identification of Ppa1 and Ppa2 proteins. An immunoblot with anti-ppa2 antibody is shown (ref.1). lane 1: Wild-type S. pombe lane 2: ppa1 lane 3: ppa2 lane 4: Wild-type carrying a multicopy plasmid with ppa1 gene lane 5: Wild-type carrying a multicopy plasmid with ppa2 gene lane 6:Wild-type carrying a multicopy plasmid with ADH promoter ligated with ppa2 gene The positions of ppa1 (36 kDa) and ppa2 (39 kDa) polypeptide bands are indicated.

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399