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Presenilin 1 antibody [C1C2], Internal

KO/KD
Anti-Presenilin 1 antibody [C1C2], Internal used in Western Blot (WB). GTX116016
Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016
Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016
Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016
Anti-Presenilin 1 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116016

Cat. No. GTX116016

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Presenilin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Presenilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037782

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

presenilin 1 , ACNINV3 , AD3 , FAD , PS-1 , PS1 , S182

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein , Golgi apparatus membrane; Multi-pass membrane protein , Cell surface

Background

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Presenilin 1 antibody [C1C2], Internal used in Western Blot (WB). GTX116016

GTX116016 WB Image

KO/KD

Wild-type (WT) and Presenilin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Presenilin 1 antibody [C1C2], Internal (GTX116016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016

GTX116016 IHC-P Image

Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Presenilin 1 antibody [C1C2], Internal (GTX116016) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016

GTX116016 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Presenilin 1(GTX116016) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016

GTX116016 IHC-P Image

Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Presenilin 1 antibody [C1C2], Internal (GTX116016) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Presenilin 1 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116016

GTX116016 ICC/IF Image

Presenilin 1 antibody [C1C2], Internal detects Presenilin 1 protein at Golgi apparatus and endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Presenilin 1 stained by Presenilin 1 antibody [C1C2], Internal (GTX116016) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

Application Reference

Ilaria Palmieri et al. Int J Mol Sci 2021; 22 (8) PSEN1 Compound Heterozygous Mutations Associated with Cerebral Amyloid Angiopathy and Cognitive Decline Phenotype.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169