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Pyruvate Carboxylase antibody

Anti-Pyruvate Carboxylase antibody used in Immunoprecipitation (IP). GTX132002
Anti-Pyruvate Carboxylase antibody used in Western Blot (WB). GTX132002
Anti-Pyruvate Carboxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX132002
Anti-Pyruvate Carboxylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132002

Cat. No. GTX132002

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, RIP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Pyruvate Carboxylase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:857
RIP Assay dependent
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Pyruvate Carboxylase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886546

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate carboxylase , PCB

Cellular Localization

Mitochondrion matrix

Background

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Carboxylase antibody used in Immunoprecipitation (IP). GTX132002

GTX132002 IP Image

Immunoprecipitation of PCB protein from HeLa whole cell extracts using 5 μg of PCB antibody (GTX132002).
Western blot analysis was performed using PCB antibody (GTX132002) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Pyruvate Carboxylase antibody used in Western Blot (WB). GTX132002

GTX132002 WB Image

PCB antibody detects PCB protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PCB antibody (GTX132002) diluted at a dilution of 1:1000.

Anti-Pyruvate Carboxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX132002

GTX132002 IHC-P Image

PCB antibody detects PCB protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
PCB antibody (GTX132002) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Pyruvate Carboxylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132002

GTX132002 ICC/IF Image

PCB antibody detects PCB protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: PCB protein stained by PCB antibody (GTX132002) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Allen SP et al. Front Aging Neurosci 2023; 15 : 1151848 A Y374X TDP43 truncation leads to an altered metabolic profile in amyotrophic lateral sclerosis fibroblasts driven by pyruvate and TCA cycle intermediate alterations.
Bolin Cai et al. Cell Death Dis 2022; 13 (4) : 389 Long noncoding RNA ZFP36L2-AS functions as a metabolic modulator to regulate muscle development.
Application : WB, RIP
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169