APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT1E3 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PKLR protein.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
pyruvate kinase L/R , PK1 , PKL , PKLR , PKR , PKRL , RPK , pyruvate kinase, liver and RBC , Pyruvate Kinase (liver/RBC)
Background
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX57581 WB Image
WB analysis of 293T whole cell lysate using GTX57581 Pyruvate Kinase (liver/RBC) antibody. Loading : 40 μg Dilution : 1:500
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GTX57581 WB Image
WB analysis of various samples using GTX57581 Pyruvate Kinase (liver/RBC) antibody. Lane 1 : Balb/3T3 whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : Raji whole cell lysate Lane 4 : K562 whole cell lysate Lane 5 : A549 whole cell lysate Loading : 40 μg Dilution : 1:500
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GTX57581 ICC/IF Image
ICC/IF analysis of Hep3B cells using GTX57581 Pyruvate Kinase (liver/RBC) antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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REFERENCE
There are currently no references for Pyruvate Kinase (liver/RBC) antibody [AT1E3] (GTX57581). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Pyruvate Kinase (liver/RBC) antibody [AT1E3] (GTX57581). Be the first to share your experience with this product.