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Pyruvate Kinase (liver/RBC) antibody

Overexpression
Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX107546
Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX107546
Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX107546
Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX107546

Cat. No. GTX107546

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Pyruvate Kinase (liver/RBC) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

K562 , Raw264.7 , rat liver , human PKLR-transfected 293T cells

Product Note

This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951259

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate kinase L/R , PK1 , PKL , PKRL , RPK

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX107546

GTX107546 WB Image

Overexpression

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 5 μg whole cell lysate/extract of human PKLR-transfected 293T cells
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107546) dilution: 1:10000

Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX107546

GTX107546 WB Image

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 30 μg Raw264.7 whole cell lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107546) dilution: 1:1000

Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX107546

GTX107546 WB Image

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 50 μg rat liver lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107546) dilution: 1:500

Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX107546

GTX107546 WB Image

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 30 μg K562 whole cell lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107546) dilution: 1:1000

REFERENCE

Application Reference

Taniguchi K et al. Int J Mol Sci 2018; 19 (5) Organ-Specific MicroRNAs (MIR122, 137, and 206) Contribute to Tissue Characteristics and Carcinogenesis by Regulating Pyruvate Kinase M1/2 (PKM) Expression.
Application : WB
Reactivity : Human
Shimada M et al. Nutrition Research 2017; (Epub) Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
Application : WB
Reactivity : Rat
Zhou X et al. Technol Cancer Res Treat 2016; 15 (5) : 697-706 Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics.
Application : WB
Reactivity : Human
Huang JY et al. RNA 2015; 21 (3) : 385-400 MicroRNA-130a can inhibit hepatitis B virus replication via targeting PGC1α and PPARγ.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169