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Pyruvate dehydrogenase kinase 1 / PDK1 antibody

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody used in Western Blot (WB). GTX22495
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody used in Western Blot (WB). GTX22495

Cat. No. GTX22495

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Human
Package
50 μg ($399)
See all Pyruvate dehydrogenase kinase 1 / PDK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:100000
IHC Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to a region near the C-terminal of human PDK-1 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate dehydrogenase kinase 1

Cellular Localization

Mitochondrion matrix

Background

PDK 1 (3-Phosphoinositide-Dependant Protein Kinase 1) phosphorylates AGC kinases. PDK 1 activates conventional PKC and PKCz (zeta) through phosphorylation of critical threonine residues in the activation loop. PDK 1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

Database

Research Area

DATA IMAGES

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody used in Western Blot (WB). GTX22495

GTX22495 WB Image

Western blot using GeneTex's affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 μg of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4º C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended. Other detection systems will yield similar results.

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody used in Western Blot (WB). GTX22495

GTX22495 WB Image

WB analysis of myc-tagged human PDK-1 protein using GTX22495 Pyruvate dehydrogenase kinase 1 / PDK1 antibody.
Dilution : 1:750

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399