APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T
Predict Reactivity
Bovine, Cat, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human QKI. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888393
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
QKI, KH domain containing RNA binding , Hqk , QK , QK1 , QK3 , hqkI
Cellular Localization
Nucleus , Cytoplasm
Background
QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998 [PubMed 9671495]). QKI proteins regulate RNA splicing, export of target RNAs from the nucleus, translation of proteins, and RNA stability (Lauriat et al., 2008 [PubMed 17918747]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-QKI antibody [GT2612] used in Western Blot (WB). GTX633921](/templates/default/js/lightbox/images/loading.gif "Anti-QKI antibody [GT2612] used in Western Blot (WB). GTX633921")
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GTX633921 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody [GT2612] (GTX633921) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX633921 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody [GT2612] (GTX633921) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX633921 IHC-P Image
QKI antibody [GT2612] detects QKI protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.QKI stained by QKI antibody [GT2612] (GTX633921) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633921 IHC-P Image
QKI antibody [GT2612] detects QKI protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.QKI stained by QKI antibody [GT2612] (GTX633921) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633921 IHC-P Image
QKI antibody [GT2612] detects QKI protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
QKI antibody [GT2612] (GTX633921) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633921 IHC-P Image
QKI antibody [GT2612] detects QKI protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
QKI antibody [GT2612] (GTX633921) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
There are currently no references for QKI antibody [GT2612] (GTX633921). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for QKI antibody [GT2612] (GTX633921). Be the first to share your experience with this product.
![Anti-QKI antibody [GT2612] used in Western Blot (WB). GTX633921](/upload/website/prouct_img/small/GTX633921/GTX633921_42709_20180817_WB_shRNA_watermark_w_23061202_966.webp "Anti-QKI antibody [GT2612] used in Western Blot (WB). GTX633921")
![Anti-QKI antibody [GT2612] used in Western Blot (WB). GTX633921](/upload/website/prouct_img/small/GTX633921/GTX633921_44713_20220610_WB_22062919_980.webp "Anti-QKI antibody [GT2612] used in Western Blot (WB). GTX633921")
![Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921](/upload/website/prouct_img/small/GTX633921/GTX633921_44713_20220715_IHC-P_M_22071823_926.webp "Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921")
![Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921](/upload/website/prouct_img/small/GTX633921/GTX633921_44713_20220715_IHC-P_R_22071823_360.webp "Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921")
![Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921](/upload/website/prouct_img/small/GTX633921/GTX633921_42709_20170405_IHC-P_R_w_23061202_269.webp "Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921")
![Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921](/upload/website/prouct_img/small/GTX633921/GTX633921_42709_20170405_IHC-P_M_w_23061202_371.webp "Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921")
![Anti-QKI antibody [GT2612] used in Western Blot (WB). GTX633921](/upload/website/prouct_img/normal/GTX633921/GTX633921_42709_20180817_WB_shRNA_watermark_w_23061202_966.webp "Anti-QKI antibody [GT2612] used in Western Blot (WB). GTX633921")
![Anti-QKI antibody [GT2612] used in Western Blot (WB). GTX633921](/upload/website/prouct_img/normal/GTX633921/GTX633921_44713_20220610_WB_22062919_980.webp "Anti-QKI antibody [GT2612] used in Western Blot (WB). GTX633921")
![Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921](/upload/website/prouct_img/normal/GTX633921/GTX633921_44713_20220715_IHC-P_M_22071823_926.webp "Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921")
![Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921](/upload/website/prouct_img/normal/GTX633921/GTX633921_44713_20220715_IHC-P_R_22071823_360.webp "Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921")
![Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921](/upload/website/prouct_img/normal/GTX633921/GTX633921_42709_20170405_IHC-P_R_w_23061202_269.webp "Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921")
![Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921](/upload/website/prouct_img/normal/GTX633921/GTX633921_42709_20170405_IHC-P_M_w_23061202_371.webp "Anti-QKI antibody [GT2612] used in IHC (Paraffin sections) (IHC-P). GTX633921")