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RAB17 antibody [N1C3]

Anti-RAB17 antibody [N1C3] used in Western Blot (WB). GTX117846
Anti-RAB17 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117846
Anti-RAB17 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117846

Cat. No. GTX117846

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RAB17 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

U87-MG , MCF-7

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619638

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB17, member RAS oncogene family

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

The Rab subfamily of small GTPases plays an important role in the regulation of membrane trafficking. RAB17 is an epithelial cell-specific GTPase (Lutcke et al., 1993 [PubMed 8486736]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RAB17 antibody [N1C3] used in Western Blot (WB). GTX117846

GTX117846 WB Image

Sample (30 μg of whole cell lysate)
A: U87-MG
B: MCF-7
12% SDS PAGE
GTX117846 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB17 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117846

GTX117846 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Rab17(GTX117846) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB17 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117846

GTX117846 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using Rab17(GTX117846) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169