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RAB26 antibody [N2C3-2]

Orthogonal
Anti-RAB26 antibody [N2C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118872
Anti-RAB26 antibody [N2C3-2] used in Western Blot (WB). GTX118872
Anti-RAB26 antibody [N2C3-2] used in Western Blot (WB). GTX118872

Cat. No. GTX118872

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all RAB26 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB26. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173841

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB26, member RAS oncogene family , V46133

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

Members of the RAB protein family, including RAB26, are important regulators of vesicular fusion and trafficking. The RAB family of small G proteins regulates intercellular vesicle trafficking, including exocytosis, endocytosis, and recycling (summary by Seki et al., 2000 [PubMed 11043516]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RAB26 antibody [N2C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118872

GTX118872 ICC/IF Image

RAB26 antibody [N2C3-2] detects RAB26 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB26 stained by RAB26 antibody [N2C3-2] (GTX118872) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RAB26 antibody [N2C3-2] used in Western Blot (WB). GTX118872

GTX118872 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX118872 diluted at 1:1000

Anti-RAB26 antibody [N2C3-2] used in Western Blot (WB). GTX118872

GTX118872 WB Image

Orthogonal

The data was published in the journal Mol Cancer in 2017.PMID: 28784136

REFERENCE

Application Reference

Chang YC et al. Mol Cancer 2017; 16 (1) : 135 Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169