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RAB27A antibody

KO/KD
Anti-RAB27A antibody used in Western Blot (WB). GTX55770
Anti-RAB27A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55770
Anti-RAB27A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55770
Anti-RAB27A antibody used in Western Blot (WB). GTX55770
Anti-RAB27A antibody used in IHC (Paraffin sections) (IHC-P). GTX55770
Anti-RAB27A antibody used in IHC (Paraffin sections) (IHC-P). GTX55770

Cat. No. GTX55770

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all RAB27A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human RAB27A (NP_899057.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB27A, member RAS oncogene family , GS2 , HsT18676 , RAB27 , RAM

Cellular Localization

Membrane,Lysosome

Background

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAB27A antibody used in Western Blot (WB). GTX55770

GTX55770 WB Image

WB analysis of U-937 cell lysate using GTX55770 RAB27A antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RAB27A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55770

GTX55770 ICC/IF Image

ICC/IF analysis of L929 cells using GTX55770 RAB27A antibody.
Blue : DAPI
Dilution : 1:100

Anti-RAB27A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55770

GTX55770 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX55770 RAB27A antibody.
Blue : DAPI
Dilution : 1:100

Anti-RAB27A antibody used in Western Blot (WB). GTX55770

GTX55770 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX55770 RAB27A antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RAB27A antibody used in IHC (Paraffin sections) (IHC-P). GTX55770

GTX55770 IHC-P Image

IHC-P analysis of human adenomyosis tissue using GTX55770 RAB27A antibody.
Dilution : 1:200

Anti-RAB27A antibody used in IHC (Paraffin sections) (IHC-P). GTX55770

GTX55770 IHC-P Image

IHC-P analysis of human liver damage tissue using GTX55770 RAB27A antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399