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RAB2B antibody

Anti-RAB2B antibody used in IHC (Paraffin sections) (IHC-P). GTX108606
Anti-RAB2B antibody used in Western Blot (WB). GTX108606
Anti-RAB2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108606
Anti-RAB2B antibody used in Western Blot (WB). GTX108606

Cat. No. GTX108606

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RAB2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human RAB2B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951570

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB2B, member RAS oncogene family

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side (Potential) , Endoplasmic reticulum membrane (Potential) , Golgi apparatus membrane

Background

Members of the Rab protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. Rab proteins are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking; see MIM 179508.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RAB2B antibody used in IHC (Paraffin sections) (IHC-P). GTX108606

GTX108606 IHC-P Image

RAB2B antibody detects RAB2B protein at cytoplasm and membrane on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma.
RAB2B antibody (GTX108606) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAB2B antibody used in Western Blot (WB). GTX108606

GTX108606 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB2B antibody (GTX108606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108606

GTX108606 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAB2B(GTX108606) antibody at 1:200 dilution.

Anti-RAB2B antibody used in Western Blot (WB). GTX108606

GTX108606 WB Image

Sample(30 ug whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX108606 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169