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RAB31 antibody

Anti-RAB31 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55929
Anti-RAB31 antibody used in Western Blot (WB). GTX55929

Cat. No. GTX55929

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RAB31 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of RAB31. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB31, member RAS oncogene family , Rab22B

Background

Small GTP-binding proteins of the RAB family, such as RAB31, play essential roles in vesicle and granule targeting (Bao et al., 2002 [PubMed 11784320]).[supplied by OMIM, Jul 2009]

Database

Research Area

DATA IMAGES

Anti-RAB31 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55929

GTX55929 ICC/IF Image

ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX55929 RAB31 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-RAB31 antibody used in Western Blot (WB). GTX55929

GTX55929 WB Image

WB analysis of MCF7 (A), Raw264.7 (B), rat brain (C) whole cell lysates using GTX55929 RAB31 antibody.

REFERENCE

Application Reference

Wei D et al. Cell Res 2020; (Epub) RAB31 marks and controls an ESCRT-independent exosome pathway.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399