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RAB33B antibody [N2C3]

Anti-RAB33B antibody [N2C3] used in Western Blot (WB). GTX116390
Anti-RAB33B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116390
Anti-RAB33B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116390
Anti-RAB33B antibody [N2C3] used in Western Blot (WB). GTX116390
Anti-RAB33B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116390
Anti-RAB33B antibody [N2C3] used in Western Blot (WB). GTX116390

Cat. No. GTX116390

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RAB33B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Mouse brain , MCF-7 , MDA-MB-231 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB33B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624273

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB33B, member RAS oncogene family , SMC2

Cellular Localization

Golgi apparatus membrane; Lipid-anchor

Background

Small GTP-binding proteins of the RAB family, such as RAB33B, play important roles at defined steps of vesicular transport in protein secretion and the endocytosis pathway (Zheng et al., 1998 [PubMed 9512502]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RAB33B antibody [N2C3] used in Western Blot (WB). GTX116390

GTX116390 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB33B antibody [N2C3] (GTX116390) diluted at 1:1000.

Anti-RAB33B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116390

GTX116390 IHC-P Image

RAB33B antibody [N2C3] detects RAB33B protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RAB33B antibody [N2C3] (GTX116390) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAB33B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116390

GTX116390 IHC-P Image

RAB33B antibody [N2C3] detects RAB33B protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RAB33B antibody [N2C3] (GTX116390) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAB33B antibody [N2C3] used in Western Blot (WB). GTX116390

GTX116390 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX116390 diluted at 1:1000

Anti-RAB33B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116390

GTX116390 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using RAB33B(GTX116390) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAB33B antibody [N2C3] used in Western Blot (WB). GTX116390

GTX116390 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB33B antibody [N2C3] (GTX116390) diluted at 1:1000.

REFERENCE

Application Reference

Lin HH et al. Biol Res 2023; 56 (1) : 7 YULINK regulates vascular formation in zebrafish and HUVECs.
Application : ICC/IF
Reactivity : Human
Henderson MX et al. J Neurosci 2017; 37 (24) : 5870-5884 Unbiased Proteomics of Early Lewy Body Formation Model Implicates Active Microtubule Affinity-Regulating Kinases (MARKs) in Synucleinopathies.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169