RAB35 antibody [N1C3]

Name: RAB35 antibody [N1C3]
Brand: GeneTex
SKU: GTX120294
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-RAB35 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120294

Anti-RAB35 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120294

Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

Cat. No. GTX120294

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Hamster
Package	100 μl ($399), 25 μl ($169)

See all RAB35 products

View product citations for antibody GTX120294 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724116

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB35, member RAS oncogene family , H-ray , RAB1C , RAY

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side (Potential) , Melanosome

Database

Gene ID: 11021 RAB35
UniProt: Q15286 RAB35

Research Area

DATA IMAGES

GTX120294 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using RAB35(GTX120294) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX120294 ICC/IF Image

RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB35 stained by RAB35 antibody [N1C3] (GTX120294) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX120294 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX120294 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX120294 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX120294 IHC-P Image

RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
RAB35 stained by RAB35 antibody [N1C3] (GTX120294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX120294 IHC-P Image

RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RAB35 stained by RAB35 antibody [N1C3] (GTX120294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX120294 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wu B et al. Carcinogenesis 2022; 43 (11) : 1059-1070 LAMTOR1 degrades MHC-II via the endocytic in hepatocellular carcinoma.

Application : WB

Reactivity : Human

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