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RAB5A antibody

KO/KD Orthogonal
Anti-RAB5A antibody used in Immunoprecipitation (IP). GTX109665
Anti-RAB5A antibody used in IHC (Paraffin sections) (IHC-P). GTX109665
Anti-RAB5A antibody used in IHC (Paraffin sections) (IHC-P). GTX109665
Anti-RAB5A antibody used in Western Blot (WB). GTX109665
Anti-RAB5A antibody used in Western Blot (WB). GTX109665
Anti-RAB5A antibody used in Western Blot (WB). GTX109665
Anti-RAB5A antibody used in Western Blot (WB). GTX109665
Anti-RAB5A antibody used in IHC (Paraffin sections) (IHC-P). GTX109665
Anti-RAB5A antibody used in Western Blot (WB). GTX109665

Cat. No. GTX109665

3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RAB5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Sheep, Bovine, Dog, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RAB5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951580

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB5A, member RAS oncogene family , RAB5

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side , Early endosome membrane; Lipid-anchor , Melanosome

Database

Research Area

DATA IMAGES

Anti-RAB5A antibody used in Immunoprecipitation (IP). GTX109665

GTX109665 IP Image

Rab5A antibody immunoprecipitates Rab5A protein in IP experiments. IP Sample: 1 mg HeLa whole cell lysate/extract A. 30 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Rab5A protein by 2 μg of Rab5A antibody (GTX109665) 15% SDS-PAGE The immunoprecipitated Rab5A protein was detected by Rab5A antibody (GTX109665) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-RAB5A antibody used in IHC (Paraffin sections) (IHC-P). GTX109665

GTX109665 IHC-P Image

RAB5A antibody detects RAB5A protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RAB5A stained by RAB5A antibody (GTX109665) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB5A antibody used in IHC (Paraffin sections) (IHC-P). GTX109665

GTX109665 IHC-P Image

RAB5A antibody detects RAB5A protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RAB5A stained by RAB5A antibody (GTX109665) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB5A antibody used in Western Blot (WB). GTX109665

GTX109665 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB5A antibody (GTX109665) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB5A antibody used in Western Blot (WB). GTX109665

GTX109665 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX109665 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB5A antibody used in Western Blot (WB). GTX109665

GTX109665 WB Image

Orthogonal

Jurkat whole cell extracts and Jurkat exosome extract (15.4 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB5A antibody (GTX109665) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB5A antibody used in Western Blot (WB). GTX109665

GTX109665 WB Image

RAB5A antibody detects RAB5A protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
12 % SDS-PAGE
RAB5A antibody (GTX109665) dilution: 1:1000

Anti-RAB5A antibody used in IHC (Paraffin sections) (IHC-P). GTX109665

GTX109665 IHC-P Image

RAB5A antibody detects RAB5A protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
RAB5A antibody (GTX109665) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAB5A antibody used in Western Blot (WB). GTX109665

GTX109665 WB Image

RAB5A antibody detects RAB5A protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
12 % SDS-PAGE
RAB5A antibody (GTX109665) dilution: 1:1000

REFERENCE

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REVIEW

RAB5A antibody Cat. No. GTX109665

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-RAB5A antibody used in Western Blot (WB). GTX109665
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and RAB5A KO cells
Amount used : 30 μg
Figure Note : Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-RAB5A antibody used in IHC (Paraffin sections) (IHC-P). GTX109665
Date : Anonymous submitted on 29-May-2023
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse Spinal cord
Blocking : 0.5% skim milk in PBST, 25°C, 2Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : Microwave heating in citrate buffer / PBS containing Triton X-100 (PBST)
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-RAB5A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109665
Date : Anonymous submitted on 17-Nov-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Hepatocytes
Blocking : 5% FBS + donkey serum in Citrate buffer, 37°C, 0.5Hr
Fixation : Acetone
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : Blue: Hoechst33342 for cell nuclei of human hepatocytes Orange: CF633 for Rab5a Image acquisition: 630X ZEISS, LSM 880 Confocal Microscope with Airyscan Thickness: 6 micrometer
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169