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RAD23A antibody

Orthogonal
Anti-RAD23A antibody used in Western Blot (WB). GTX23836
Anti-RAD23A antibody used in Western Blot (WB). GTX23836

Cat. No. GTX23836

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all RAD23A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:4000-1:16000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region near aa 115-140 of human HR23A protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD23 homolog A, nucleotide excision repair protein , HHR23A , HR23A

Cellular Localization

Nucleus

Background

HHR23A was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Database

Research Area

DATA IMAGES

Anti-RAD23A antibody used in Western Blot (WB). GTX23836

GTX23836 WB Image

Western blot showing GeneTex's Affinity Purified anti-HR23A antibody detects endogenous human HR23A. Reactivity is shown against HeLa nuclear extract (lane 1) and HeLa (lane 2), A431 (lane 3), Jurkat (lane 4) and 293 whole cell lysates (lane 5). Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG and chemilumi-nescence reagent with a 30-min exposure time. Other detection systems will yield similar results.

Anti-RAD23A antibody used in Western Blot (WB). GTX23836

GTX23836 WB Image

Orthogonal

WB analysis of various samples using GTX23836 RAD23A antibody.
Lane 1 : HeLa nuclear extract
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : 293T whole cell lysate
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399