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RAD23B antibody

Anti-RAD23B antibody used in IHC (Paraffin sections) (IHC-P). GTX85831
Anti-RAD23B antibody used in Western Blot (WB). GTX85831

Cat. No. GTX85831

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
25 μg ($399)
See all RAD23B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 2.5 μg/ml
ELISA 1:2000 - 1:10000

Note :

IHC-P
Heat induced antigen retrieval in pH 6.0 citrate buffer
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide from human RAD23B.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD23 homolog B, nucleotide excision repair protein , HHR23B , HR23B , P58 , RAD23B , Rad23B , hHR23b , HR23b

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-RAD23B antibody used in IHC (Paraffin sections) (IHC-P). GTX85831

GTX85831 IHC-P Image

IHC-P analysis of human prostate tissue using GTX85831 RAD23B antibody.
Antigen retrieval : Heat-induced antigen retrieval
Dilution : 5 μg/ml

Anti-RAD23B antibody used in Western Blot (WB). GTX85831

GTX85831 WB Image

WB analysis of HeLa whole cell lysate using GTX85831 RAD23B antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399