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RAD51AP1 antibody [N1C1]

Anti-RAD51AP1 antibody [N1C1] used in Western Blot (WB). GTX115455
Anti-RAD51AP1 antibody [N1C1] used in Western Blot (WB). GTX115455
Anti-RAD51AP1 antibody [N1C1] used in Western Blot (WB). GTX115455
Anti-RAD51AP1 antibody [N1C1] used in Western Blot (WB). GTX115455

Cat. No. GTX115455

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RAD51AP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAD51AP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729638

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD51 associated protein 1 , PIR51

Background

May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.

Database

Research Area

DATA IMAGES

Anti-RAD51AP1 antibody [N1C1] used in Western Blot (WB). GTX115455

GTX115455 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RAD51AP1 antibody [N1C1] (GTX115455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RAD51AP1 antibody [N1C1] used in Western Blot (WB). GTX115455

GTX115455 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAD51AP1 antibody [N1C1] (GTX115455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAD51AP1 antibody [N1C1] used in Western Blot (WB). GTX115455

GTX115455 WB Image

The data was published in the journal Nat Commun in 2019.PMID: 31844045

Anti-RAD51AP1 antibody [N1C1] used in Western Blot (WB). GTX115455

GTX115455 WB Image

The data was published in the journal Nat Commun in 2019.PMID: 31844045

REFERENCE

Application Reference

Bruno Silva et al. Nat Commun 2021; 12 (1) : 3760 TERRA transcription destabilizes telomere integrity to initiate break-induced replication in human ALT cells.
Application : WB
Reactivity : Human
Park SH et al. Nat Commun 2019; 10 (1) : 5718 ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169