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RAD9A antibody [3A3-A7-F8]

Anti-RAD9A antibody [3A3-A7-F8] used in Immunoprecipitation (IP). GTX49140
Anti-RAD9A antibody [3A3-A7-F8] used in Western Blot (WB). GTX49140

Cat. No. GTX49140

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A3-A7-F8

Isotype

 IgG2b

Application

 WB, IP

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μg ($399)
See all RAD9A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human Rad9A protein fragments expressed in E.coli

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD9 checkpoint clamp component A , RAD9

Cellular Localization

Nucleus

Background

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-RAD9A antibody [3A3-A7-F8] used in Immunoprecipitation (IP). GTX49140

GTX49140 IP Image

IP analysis of 3T3 and HeLa lysates using control IgG and RAD9A antibody. The precipitates were detected by the same RAD9A antibody.

Anti-RAD9A antibody [3A3-A7-F8] used in Western Blot (WB). GTX49140

GTX49140 WB Image

WB analysis of indicated lysates using RAD9A antibody at a dilution of 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399