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RAG2 antibody

Anti-RAG2 antibody used in Western Blot (WB). GTX109710
Anti-RAG2 antibody used in Western Blot (WB). GTX109710
Anti-RAG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109710
Anti-RAG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX109710

Cat. No. GTX109710

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all RAG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Molt-4 , Rat thymus

Predict Reactivity

Mouse, Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951618

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

recombination activating 2 , RAG-2

Cellular Localization

Nucleus

Background

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RAG2 antibody used in Western Blot (WB). GTX109710

GTX109710 WB Image

RAG2 antibody detects RAG2 protein by Western blot analysis.
A. 50 μg Rat thymus lysate/extract
7.5 % SDS-PAGE
RAG2 antibody (GTX109710) dilution: 1:1000

Anti-RAG2 antibody used in Western Blot (WB). GTX109710

GTX109710 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109710 diluted at 1:1000

Anti-RAG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109710

GTX109710 ICC/IF Image

RAG2 antibody detects RAG2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAG2 protein stained by RAG2 antibody (GTX109710) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-RAG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX109710

GTX109710 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using RAG2(GTX109710) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169