GeneTex

  • Country / Location Selection

United States (US)

RAG2 antibody

KO/KD
Anti-RAG2 antibody used in Western Blot (WB). GTX54683
Anti-RAG2 antibody used in Western Blot (WB). GTX54683
Anti-RAG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54683
Anti-RAG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54683

Cat. No. GTX54683

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all RAG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2 (NP_001230715.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

recombination activating 2 , RAG-2

Cellular Localization

Nucleus

Background

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAG2 antibody used in Western Blot (WB). GTX54683

GTX54683 WB Image

WB analysis of various sample lysates using GTX54683 RAG2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RAG2 antibody used in Western Blot (WB). GTX54683

GTX54683 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX54683 RAG2 antibody.
Dilution : 1:500
Loading : 25μg per lane

Anti-RAG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54683

GTX54683 ICC/IF Image

ICC/IF analysis of GFP-RNF168 transgenic U2OS cells using GTX54683 RAG2 antibody.
Green : GFP-RNF168 fusion protein expression for DNA damage marker
Blue : DAPI

Anti-RAG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54683

GTX54683 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54683 RAG2 antibody.
Blue : DAPI

REFERENCE

There are currently no references for RAG2 antibody (GTX54683). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for RAG2 antibody (GTX54683). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399