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RALBP1 antibody [11B2]

Overexpression
Anti-RALBP1 antibody [11B2] used in Western Blot (WB). GTX83728
Anti-RALBP1 antibody [11B2] used in Western Blot (WB). GTX83728
Anti-RALBP1 antibody [11B2] used in Flow cytometry (FACS). GTX83728
Anti-RALBP1 antibody [11B2] used in Flow cytometry (FACS). GTX83728
Anti-RALBP1 antibody [11B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83728
Anti-RALBP1 antibody [11B2] used in Immunoprecipitation (IP). GTX83728

Cat. No. GTX83728

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 11B2

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IP

Reactivity

 Human, Rat, Dog
Package
100 μl ($519)
See all RalBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human RALBP1 (NP_006779) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ralA binding protein 1 , RIP1 , RLIP1 , RLIP76

Cellular Localization

Membrane

Background

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.

Database

Research Area

DATA IMAGES

Anti-RALBP1 antibody [11B2] used in Western Blot (WB). GTX83728

GTX83728 WB Image

Overexpression

WB analysis of HEK293T cells transfected with RALBP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83728 RALBP1 antibody [11B2].
Loading : 5 ug per lane

Anti-RALBP1 antibody [11B2] used in Western Blot (WB). GTX83728

GTX83728 WB Image

WB analysis of various cell lines using GTX83728 RALBP1 antibody [11B2].
Loading : 35 ug per lane

Anti-RALBP1 antibody [11B2] used in Flow cytometry (FACS). GTX83728

GTX83728 FACS Image

FACS analysis of HEK293T cells transfected with either RALBP1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83728 RALBP1 antibody [11B2].

Anti-RALBP1 antibody [11B2] used in Flow cytometry (FACS). GTX83728

GTX83728 FACS Image

FACS analysis of Jurkat cells using GTX83728 RALBP1 antibody [11B2].
Red : Primary antibody
Blue : Negative control antibody

Anti-RALBP1 antibody [11B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83728

GTX83728 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with RALBP1 plasmid using GTX83728 RALBP1 antibody [11B2].

Anti-RALBP1 antibody [11B2] used in Immunoprecipitation (IP). GTX83728

GTX83728 IP Image

IP analysis of DDDDK tagged RALBP1 overexpressed HEK293T lysate using GTX83728 RALBP1 antibody [11B2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519