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RAMP2 antibody

Orthogonal
Anti-RAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX108524
Anti-RAMP2 antibody used in Western Blot (WB). GTX108524
Anti-RAMP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108524

Cat. No. GTX108524

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RAMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RAMP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951626

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

receptor activity modifying protein 2

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX108524

GTX108524 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using RAMP2(GTX108524) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAMP2 antibody used in Western Blot (WB). GTX108524

GTX108524 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAMP2 antibody (GTX108524) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAMP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108524

GTX108524 IHC-Fr Image

The data was published in the journal Mol Vis in 2012.PMID: 22690112

REFERENCE

Application Reference

Edvinsson L et al. J Mol Neurosci 2020; 70 (6) : 930-944 Expression of the CGRP Family of Neuropeptides and their Receptors in the Trigeminal Ganglion.
Blom J et al. Mol Vis 2012; 18 : 1339-53 Evidence for a functional adrenomedullin signaling pathway in the mouse retina.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169