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RAP1 antibody [N2C3]

Anti-RAP1 antibody [N2C3] used in Western Blot (WB). GTX101983
Anti-RAP1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX101983
Anti-RAP1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101983
Anti-RAP1 antibody [N2C3] used in Western Blot (WB). GTX101983
Anti-RAP1 antibody [N2C3] used in Immunoprecipitation (IP). GTX101983

Cat. No. GTX101983

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617907

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TERF2 interacting protein , DRIP5 , RAP1

Cellular Localization

Nucleus , Telomere

Background

The gene encodes a protein that is part of a complex involved in telomere length regulation. Pseudogenes are present on chromosomes 5 and 22. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RAP1 antibody [N2C3] used in Western Blot (WB). GTX101983

GTX101983 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP1 antibody [N2C3] (GTX101983) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAP1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX101983

GTX101983 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using RAP1(GTX101983) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAP1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101983

GTX101983 ICC/IF Image

RAP1 antibody [N2C3] detects RAP1 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAP1 protein stained by RAP1 antibody [N2C3] (GTX101983) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RAP1 antibody [N2C3] used in Western Blot (WB). GTX101983

GTX101983 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101983 diluted at 1:1000

Anti-RAP1 antibody [N2C3] used in Immunoprecipitation (IP). GTX101983

GTX101983 IP Image

RAP1 antibody immunoprecipitates RAP1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of RAP1 by 3 μg of RAP1 antibody (GTX101983) 10% SDS-PAGE The immunoprecipitated RAP1 protein was detected by RAP1 antibody (GTX101983) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

G Ma et al. BMC Complement Med Ther 2024; 24 (1) : 53 Network pharmacology and in vivo evidence of the pharmacological mechanism of geniposide in the treatment of atherosclerosis
Application : IHC-P
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169