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RAP1B antibody

Overexpression
Anti-RAP1B antibody used in Western Blot (WB). GTX111933
Anti-RAP1B antibody used in Western Blot (WB). GTX111933

Cat. No. GTX111933

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RAP1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Molt-4 , RAP1B-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Drosophila, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human RAP1B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951632

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAP1B, member of RAS oncogene family , K-REV , RAL1B

Cellular Localization

Cell membrane , Cytoplasm , cytosol

Background

RAP1B and RAP1A (MIM 179520) belong to a superfamily of RAS (see MIM 190020)-like small GTP-binding proteins involved in cell signaling.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RAP1B antibody used in Western Blot (WB). GTX111933

GTX111933 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
12% SDS PAGE
GTX111933 diluted at 1:1000

Anti-RAP1B antibody used in Western Blot (WB). GTX111933

GTX111933 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAP1B antibody (GTX111933) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Shen K et al. FEBS J 2014; A Post-Transcriptional Regulation of Tumor Suppressor MiR-139-5p and A Network of MiR-139-5p Mediated mRNAs Interactions in Colorectal Cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169