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RBM20 antibody

Anti-RBM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17481
Anti-RBM20 antibody used in Western Blot (WB). GTX17481

Cat. No. GTX17481

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all RBM20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
ICC/IF 10μg/ml

Note :

ICC/IF
Expression of the protein seen in the Nucleus and Golgi apparatus of U2OS cells.
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX17481-PEP).

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QLDKAELDERP, from the internal region of the protein sequence according to NP_001127835.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding motif protein 20

Cellular Localization

Nucleus

Background

This gene encodes a protein that binds RNA and regulates splicing. Mutations in this gene have been associated with familial dilated cardiomyopathy. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-RBM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17481

GTX17481 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX17481 RBM20 antibody.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-RBM20 antibody used in Western Blot (WB). GTX17481

GTX17481 WB Image

WB analysis of mouse heart lysate with (B) and without (A) blocking with the immunising peptide using GTX17481 RBM20 antibody.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399