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RBP1 antibody

Anti-RBP1 antibody used in Western Blot (WB). GTX108016
Anti-RBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108016
Anti-RBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108016
Anti-RBP1 antibody used in Western Blot (WB). GTX108016

Cat. No. GTX108016

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

NIH-3T3 , C2C12

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human RBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951663

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

retinol binding protein 1 , CRABP-I , CRBP , CRBP1 , CRBPI , RBPC

Cellular Localization

Cytoplasm

Background

This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RBP1 antibody used in Western Blot (WB). GTX108016

GTX108016 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBP1 antibody (GTX108016) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108016

GTX108016 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon, using CRBP I(GTX108016) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108016

GTX108016 ICC/IF Image

RBP1 antibody detects RBP1 protein at cytoplasm by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBP1 stained by RBP1 antibody (GTX108016) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-RBP1 antibody used in Western Blot (WB). GTX108016

GTX108016 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RBP1 antibody (GTX108016) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169