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RCBTB1 antibody [N1C1]

Anti-RCBTB1 antibody [N1C1] used in Western Blot (WB). GTX116302
Anti-RCBTB1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116302
Anti-RCBTB1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116302
Anti-RCBTB1 antibody [N1C1] used in Western Blot (WB). GTX116302

Cat. No. GTX116302

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RCBTB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

Mouse brain , U87-MG

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RCBTB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618708

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RCC1 and BTB domain containing protein 1 , CLLD7 , CLLL7 , GLP , RDEOA

Cellular Localization

Nucleus

Background

This gene encodes a protein with an N-terminal RCC1 domain and a C-terminal BTB (broad complex, tramtrack and bric-a-brac) domain. In rat, over-expression of this gene in vascular smooth muscle cells induced cellular hypertrophy. In rat, the C-terminus of RCBTB1 interacts with the angiotensin II receptor-1A. In humans, this gene maps to a region of chromosome 13q that is frequently deleted in B-cell chronic lymphocytic leukemia and other lymphoid malignancies. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RCBTB1 antibody [N1C1] used in Western Blot (WB). GTX116302

GTX116302 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX116302 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RCBTB1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116302

GTX116302 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using RCBTB1(GTX116302) antibody at 1:200 dilution.

Anti-RCBTB1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116302

GTX116302 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using RCBTB1(GTX116302) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RCBTB1 antibody [N1C1] used in Western Blot (WB). GTX116302

GTX116302 WB Image

Sample (30 μg of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX116302 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169