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REST antibody

KO/KD Orthogonal
Anti-REST antibody used in Western Blot (WB). GTX129144
Anti-REST antibody used in IHC (Paraffin sections) (IHC-P). GTX129144
Anti-REST antibody used in Western Blot (WB). GTX129144
Anti-REST antibody used in Western Blot (WB). GTX129144

Cat. No. GTX129144

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all REST products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

HeLa nuclear , U87-MG nuclear extract , HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human REST. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885909

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RE1 silencing transcription factor , DFNA27 , GINGF5 , HGF5 , NRSF , WT6 , XBR

Cellular Localization

Nucleus

Background

This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-REST antibody used in Western Blot (WB). GTX129144

GTX129144 WB Image

Orthogonal

U87-MG whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with REST antibody (GTX129144) diluted at 1:500.

Anti-REST antibody used in IHC (Paraffin sections) (IHC-P). GTX129144

GTX129144 IHC-P Image

REST antibody detects REST protein at cytoplasm and nucleus in human lung by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
REST antibody (GTX129144) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-REST antibody used in Western Blot (WB). GTX129144

GTX129144 WB Image

KO/KD

Wild-type (WT) and REST knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with REST antibody (GTX129144) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-REST antibody used in Western Blot (WB). GTX129144

GTX129144 WB Image

Orthogonal

REST antibody detects REST protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
5 % SDS-PAGE
REST antibody (GTX129144) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169